4. High Performance Liquid Chromatograph












Make: SHIMADZU, JAPAN

Model: LC-8A, 1995

Specification:

  • Flow rate accuracy: 2% (0.5-150 mL/min)
  • Flow rate stability: 0.5% (0.5-150 mL/min)
  • Wet parts material: Stainless steel, Hastelloy C, Vespel, Sappire
  • Suction filter: 10 um
  • Dimensions (mm): 350(w) x 450(d) x 210(h)

    Accessories

    User-friendly single point control from an SCL-10A controller.

    Description

    High-Performance Liquid Chromatograph (HPLC) is an analytical instrument used to separate, identify, and quantify each component in a mixture. It relies on pumps to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid adsorbent material. Each component in the sample interacts slightly differently with the adsorbent material, causing different flow rates for the different components and leading to the separation of the components as they flow out the column.

    Principle

    The sample is being injected through analytical port into the mobile phase stream which carries it into the column. The pumps deliver the desired flow and composition of the mobile phase through the column. Chromatography can be described as a mass transfer process involving adsorption. The active component of the column, the adsorbent, is typically a granular material made of solid particles (e.g. silica, polymers, etc.), 2–50 μm in size. The components of the sample mixture are separated from each other due to their different degrees of interaction with the adsorbent particles. The detector generates a signal proportional to the amount of sample component emerging from the column, hence allowing for quantitative analysis of the sample components. A digital microprocessor and user software control the HPLC instrument and provide data analysis.

    Applications

    Determination of following parameters in Tobacco:
  • Solanesol
  • Imidacloprid

    User instructions

  • Clearly mention the name of the character for which analysis is required
  • Provide minimum 0.5-1.0 kg leaf or 100-200g powder

    Analysis Charges :

  • Rs.2500/- +GST per sample for Solanesol
  • Rs.7000/- +GST per sample for Imidacloprid